Are you looking for a highly stable antibody that can bind to hard to reach active sites?

Heat Stable

Single Domain Antibodies are heat stable up to approximately seventy degrees centigrade.

pH Stable

Single Domain Antibodies are stable over a much larger pH range than traditional antibodies.

Small Size

Compared to traditional antibodies which are approximately 150 kDa in size, they are only 15 kDa.

Single Domain Antibodies can easily be converted into different formats such as Fc-fusion proteins and heterodimers. 

Easily Linked

Less Immunotoxicity

Single Domain Antibodies are less likely to be immunotoxic.

Better Penetration

Single Domain Antibodies can penetrate into tissue much more rapidly than traditional antibodies.

Use in Crystallization

Better Clearance

Single Domain Antibodies are able to pass the renal filter resulting in their rapid blood clearance.

Better Delivery

Single Domain Antibodies are more suitable for oral delivery and use in diagnostic devices.

Use as Intrabodies

Single Domain Antibodies can be used as intrabodies to target intracellular proteins.

First we start by immunizing llamas with the immunogen.

The llamas then go through a 90 day schedule during which they are boosted and bled to determine their specific anti-sera titer.

Once the anti-sera titre up, the peripheral blood mononuclear cells, also called PBMCs, are isolated and total RNA is isolated from them, and cDNA is prepared.

The VHH genes are then amplified from cDNA using proprietary PCR primers that cover the genetic diversity in this complex region.

Next, the PCR product is cloned into a phagemid vector creating the VHH antibody library.

After that the library is screened through multiple rounds of affinity panning, and positive clones are identified via ELISA, and then sequenced.

Finally, these clones are used to produce expressed, purified, llama monoclonal Single Domain Antibodies.

At Abcore we give you the unique choice of selecting one of 6 single-domain antibody production options

Linda Poplin

Jennifer Johnson

David Barraclough

Farm Technician

Operations

Client Care

Linda Poplin, Farm Tech at Abcore LLC, has been involved in the care of research animals for over 20 years and oversees the animal care program. She has extensive experience working in the San Diego biotech hub, is an expert in animal health and procedures, and was instrumental in developing proprietary procedures at Abcore. She has a genuine interest in the total care of all animals.  We are lucky to have her be a part of the Abcore team. 

Jennifer Johnson, Vice President of Operations at Abcore LLC, has 18 years of laboratory experience offering a wide variety of lab related expertise. Her specialties include polyclonal antibody production in a variety of species, ascites production, antibody purification, monoclonal antibody production / development, and project management. She has been involved in all levels of antibody production and understands the detailed processes.

David Barraclough, Client Care at Abcore LLC, has a degree in Molecular Biology from UC San Diego, where he worked as a research associate for 7 years. While working at the UC San Diego Medical School for the Howard Hughes Medical Institute he was a co-author on publications and managed the DNA sequencing facility and microscopy core facility. David also founded RenderGen, a 3D computer animation company which produced “mechanism of action” movies for pharmaceutical and biotechnology companies.

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Single Domain Antibodies can be used as crystallization chaperones in the structure determination of challenging target proteins. 

 peptide • protein • electroporation • small molecules • cell-surface proteins • plasmids • cell based immunizations • small haptens

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