ARE YOU LOOKING FOR A HIGHLY STABLE ANTIBODY THAT CAN BIND TO HARD TO REACH ACTIVE SITES?

AT ABCORE, WE PROVIDE

Single Domain Antibodies

to research scientists and companies involved with the antibody reagents market faster and more reliably than any other company.

Abcore has the following licenses:    USDA #93-R-0541    •    NIH/OLAW #A4713-01

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ABCORE'S SINGLE DOMAIN ANTIBODIES

Benefits

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HEAT STABLE
Stable up to approximately
70 degrees centigrade.
PH stable
SMALL SIZE
PH STABLE
Stable over a larger pH range
than traditional antibodies.
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Single Domain Antibodies are only 15kDa
compared to 150 kDa
traditional antibodies.
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BETTER PENETRATION
Single Domain Antibodies penetrate
into tissue more rapidly than
traditional antibodies.
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EASILY LINKED
Can easily be converted to
different formats such as Fc-fusion
proteins and heterodimers.
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BETTER DELIVERY
More suitable for use
in diagnostic devices.
intrabodies
USE AS INTRABODIES
Single Domain Antibodies can be
used as intrabodies to
target intracellular proteins.
humanized
EASILY HUMANIZED
Single Domain Antibodies are easily
humanized for pharmaceutical
applications.
First we start by immunizing llamas and or alpacas with the immunogen.
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ABCORE'S Production

Process

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The camelids then go through a 90 day schedule during which they are boosted and bled to determine their specific anti-sera titer.
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Once the anti-sera titer up, the peripheral blood mononuclear cells, also called PBMCs, are isolated and RNA is isolated from them, and cDNA is prepared.
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The VHH genes are then amplified from cDNA using proprietary, species-specific primers that cover the genetic diversity in this complex region.
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Next, the PCR product is cloned into a phagemid vector creating the VHH antibody library.
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This library undergoes multiple rounds of monitored phage panning to enrich single domain antibodies.
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The enriched phage pools are then screened to identify single domain antibodies that meet the required specificity and characteristics.
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Finally, the positive hits are sequenced to determine the single domain antibody sequence.
ABCORE'S 

Approach

STRATEGY

We collect PBMC cells early in the immunization to capture a higher diversity of binders, as well as later in the immunization where in-vivo clonal expansion of B-cells can result in higher affinities

We use proprietary, species-specific primers that have higher coverage than published primers

ANTIGEN EXPERIENCE

We have experience with many antigens such as proteins, peptides, cells, DNA, MAP peptides, liposomes, VLPS and small chemical haptens up to 120kd protein complexes.

We have successfully generated single domain antibodies against ion channels, GPCRs and small peptides including biologically active and pH dependent single domain antibodies

Variety

We can do serum titers and screening by ELISA or flow cytometry and perform phage panning and screening 4 different ways:


1. Solid-phase panning and screening antigen is bound to high-bind plastic ELISA plates. 2. Semi-solution panning and screening ibiotinylated antigen is bound to streptavidin plates. 3. Solution panning and screening biotinylated antigen is bound to streptavidin magnetic beads. 4. Live cell panning and screening performed with live cells and a control cell line.

LIBRARY DIVERSITY

We can immunize llama, alpaca, or a combination thereof to increase diversity of the library

TRANSPARENCY

Abcore's customers receive the sequence and rights to all sequences we discover. We do not hold back any positive clones discovered.

CONTINUED SUCCESS

At Abcore, you receive the comfort of working with someone who has successfully completed hundreds of single domain antibody projects, including working with 5 of the top 10 largest pharma companies in the world

ABCORE'S RODUCTION

Options

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CAMELID PBMC CELLS

Llama or alpaca PBMC cells either cryopreserved or stored in RNAlater

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RNA

RNA isolated from PBMC cells

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SDA ANTIBODY LIBRARY

Single-domain antibody phage library

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POSITIVE SEQUENCES

List of all positive single domain antibody sequences after panning and screening

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PURIFIED SINGLE DOMAIN ANTIBODIES

Expressed and purified single domain antibodies

ABCORE'S

Management Team

Jennifer Johnson
SAM SUGERMAN
DAVID BARRACLOUGH
JENNIFER JOHNSON
Operations
Jennifer Johnson, Vice President of Operations at Abcore LLC, has 18 years of laboratory experience offering a wide variety of lab related expertise. Her specialties include polyclonal antibody production in a variety of species, ascites production, antibody purification, monoclonal antibody production / development, and project management. She has been involved in all levels of antibody production and understands the detailed processes.
DAVID BARRACLOUGH
Client Care
David Barraclough, Client Care at Abcore LLC, has a degree in Molecular Biology from UC San Diego, where he worked as a research associate for 7 years. While working at the UC San Diego Medical School for the Howard Hughes Medical Institute he was a co-author on publications and managed the DNA sequencing facility and microscopy core facility. David also founded RenderGen, a 3D computer animation company which produced “mechanism of action” movies for pharmaceutical and biotechnology companies.
SAM SUGERMAN
Sr. Scientist  /  Laboratory Manager
Sam has a Ph.D. from the University of Arizona focused on protein engineering of novel and selective protein-protein interfaces. Sam comes to Abcore after working with Encodia in San Diego. Sam brings a very robust set of skills, with a focus in protein engineering and phage display. He has a focused knowledge of phage display and library design. Sam also has a strong background in structurebased and sequence-based rational designs of interactions, including
PSSM and sequence homology strategies, to make novel splitprotein systems and selective mutant interfaces.

Contact

Abcore, Inc. 

405 Maple St, Ramona, CA 92065

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c 2023 Abcore, Inc. All Rights Reserved

Abcore has the following licenses:    
USDA #93-R-0541 
NIH/OLAW #A4713-01

Abcore is now a Fortis Life Sciences Company.  

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