ARE YOU LOOKING FOR A HIGHLY STABLE ANTIBODY THAT CAN BIND TO HARD TO REACH ACTIVE SITES?
AT ABCORE, WE PROVIDE
Single Domain Antibodies
to research scientists and companies involved with the antibody reagents market faster and more reliably than any other company.
Abcore has the following licenses: USDA #93-R-0541 • NIH/OLAW #A4713-01
ABCORE'S SINGLE DOMAIN ANTIBODIES
Benefits
HEAT STABLE
Stable up to approximately 70 degrees centigrade.
SMALL SIZE
PH STABLE
Stable over a larger pH range than traditional antibodies.
Single Domain Antibodies are only 15kDa compared to 150 kDa traditional antibodies.
BETTER PENETRATION
Single Domain Antibodies penetrate into tissue more rapidly than traditional antibodies.
EASILY LINKED
Can easily be converted to different formats such as Fc-fusion proteins and heterodimers.
BETTER DELIVERY
More suitable for use in diagnostic devices.
USE AS INTRABODIES
Single Domain Antibodies can be used as intrabodies to target intracellular proteins.
EASILY HUMANIZED
Single Domain Antibodies are easily humanized for pharmaceutical applications.
First we start by immunizing llamas and or alpacas with the immunogen.
ABCORE'S Production
Process
The camelids then go through a 90 day schedule during which they are boosted and bled to determine their specific anti-sera titer.
Once the anti-sera titer up, the peripheral blood mononuclear cells, also called PBMCs, are isolated and RNA is isolated from them, and cDNA is prepared.
The VHH genes are then amplified from cDNA using proprietary, species-specific primers that cover the genetic diversity in this complex region.
Next, the PCR product is cloned into a phagemid vector creating the VHH antibody library.
This library undergoes multiple rounds of monitored phage panning to enrich single domain antibodies.
The enriched phage pools are then screened to identify single domain antibodies that meet the required specificity and characteristics.
Finally, the positive hits are sequenced to determine the single domain antibody sequence.
ABCORE'S
Approach
STRATEGY
We collect PBMC cells early in the immunization to
capture a higher diversity of binders, as well
as later in the immunization where in-vivo clonal
expansion of B-cells can result in higher
affinities
We use proprietary, species-specific primers that have
higher coverage than published primers
ANTIGEN EXPERIENCE
We have experience with many antigens such
as proteins, peptides, cells, DNA, MAP peptides,
liposomes, VLPS and small chemical haptens up
to 120kd protein complexes.
We have successfully generated single domain
antibodies against ion channels, GPCRs and
small peptides including biologically active
and pH dependent single domain antibodies
Variety
We can do serum titers and screening by ELISA or flow cytometry and perform phage panning and screening 4 different ways:
1. Solid-phase panning and screening
antigen is bound to high-bind plastic ELISA plates.
2. Semi-solution panning and screening
ibiotinylated antigen is bound to streptavidin plates.
3. Solution panning and screening
biotinylated antigen is bound to streptavidin magnetic beads.
4. Live cell panning and screening
performed with live cells and a control cell line.
LIBRARY DIVERSITY
We can immunize llama, alpaca,
or a combination thereof to increase
diversity of the library
TRANSPARENCY
Abcore's customers receive the sequence and rights
to all sequences we discover. We do not hold
back any positive clones discovered.
CONTINUED SUCCESS
At Abcore, you receive the comfort of working
with someone who has successfully completed
hundreds of single domain antibody projects,
including working with 5 of the top 10 largest
pharma companies in the world
ABCORE'S RODUCTION
Options
CAMELID PBMC CELLS
Llama or alpaca PBMC cells either cryopreserved or stored in RNAlater
RNA
RNA isolated from PBMC cells
SDA ANTIBODY LIBRARY
Single-domain antibody
phage library
POSITIVE SEQUENCES
List of all positive single domain antibody sequences after panning and screening
PURIFIED SINGLE DOMAIN ANTIBODIES
Expressed and purified single domain antibodies
ABCORE'S
Management Team
JENNIFER JOHNSON
Operations
Jennifer Johnson, Vice President of Operations at Abcore LLC, has 18 years of laboratory experience offering a wide variety of lab related expertise. Her specialties include polyclonal antibody production in a variety of species, ascites production, antibody purification, monoclonal antibody production / development, and project management. She has been involved in all levels of antibody production and understands the detailed processes.
DAVID BARRACLOUGH
Client Care
David Barraclough, Client Care at Abcore LLC, has a degree in Molecular Biology from UC San Diego, where he worked as a research associate for 7 years. While working at the UC San Diego Medical School for the Howard Hughes Medical Institute he was a co-author on publications and managed the DNA sequencing facility and microscopy core facility. David also founded RenderGen, a 3D computer animation company which produced “mechanism of action” movies for pharmaceutical and biotechnology companies.
SAM SUGERMAN
Sr. Scientist / Laboratory Manager
Sam has a Ph.D. from the University of Arizona focused on protein engineering of novel and selective protein-protein interfaces. Sam comes to Abcore after working with Encodia in San Diego. Sam brings a very robust set of skills, with a focus in protein engineering and phage display. He has a focused knowledge of phage display and library design. Sam also has a strong background in structurebased and sequence-based rational designs of interactions, including PSSM and sequence homology strategies, to make novel splitprotein systems and selective mutant interfaces.
